RNA Preparation and Purification
![Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 x 1 - packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-R2061DIRECTZOLRNAMICROPREPYES.JPG-250.jpg)
Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 x 1 - packaged separately]
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
New England Biolabs, Inc. Monarch® Spin Columns S2B and Tubes - 100 preps
The Monarch Spin Columns S2B and Tubes are a component of Monarch kits for RNA cleanup, and also offered separately for convenience and flexibility. The columns are designed without the use of a retaining ring, helping to ensure no buffer retention and no carryover of contaminants.
- Monarch spin columns and collection tubes designed for nucleic acid purification
- Minimal to no buffer retention/risk of carry-over contamination
- Low elution volume from unique column design
- Included in Monarch kits, also offered separately for flexibility
- Made with less plastic than conventional columns from leading suppliers
Norgen Biotek Corp Plant/Fungi Tot Rna Our 50 Pre
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient. The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
Molecular Research Center RNAZOL RT REAGENT 200ML
A single-step reagent isolating mRNA and small RNA in separate fractions. No chloroform-induced phase separation or refrigerated centrifuge required. No DNase treatment. Higher yields and quality than previous single-step methods.
Omega Biotek Inc E.Z.N.A. HP Total RNA Kit, 200 preps, RNA binding capacity 100 μg (RNA >200 bases), silica-based spin column
The OMEGA BIOTEK E.Z.N.A. HP Total RNA Kit provides an efficient solution for the isolation of high-quality total RNA from eukaryotic cells and tissues. It employs a streamlined purification process, integrating sample homogenization and genomic DNA removal into a single step, utilizing advanced column technology. This kit enhances user safety by eliminating toxic organic solvents and simplifies the protocol for RNA purification, making it suitable for various sensitive applications.
- Rapid isolation in approximately 25 minutes for quick experimental turnaround
- High-performance RNA quality and yield
- Versatile compatibility with various cell and tissue types
- Safe and convenient with no toxic organic solvents
- Optimized for sensitive downstream applications like RT-PCR and next-generation sequencing
- High binding capacity of 100 μg for RNA >200 bases
BIOSEARCH TECHNOLOGIES INC (Lucigen) MasterPure Yeast RNA Purification Kit, 100 PurificationsSafe, fast purification of high quality RNA from multiple species of yeast.
The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi.
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (10 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (10 ug) rapidly and reliably purifies up to 10 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. The kit can also be applied for total RNA extraction from some sample types. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 ul. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 50 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
System Biosciences LLC SeraMir Exosome RNA Purification Column Kit (20 exoRNA columns), 20 Preps
Efficiently purify RNA from already isolated exosomes with SBI’s most flexible SeraMir Kit.
Molecular Research Center RNAzol RT, 500 mL
RNAzol RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.
Clontech Labs 3P NucleoSpin RNA Blood Midi | 20 Preps
The NucleoSpin RNA Blood Midi kit enables efficient recovery of high-quality RNA from up to 1.3 ml of whole blood using a simple and convenient procedure that can be performed at room temperature. The kit protocol is compatible with common blood collection tubes and anticoagulants—including EDTA, citrate, and heparin—and enables processing of 6 samples in 75 minutes.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Heart, Ambient temperature shipping, 50 ug/pk
Total RNA is isolated by modified guanidine thiocyanate techniques and stored in RNA storage buffer. The vials were then dried for room temperature shipping and storage. The total RNA need to be reconsitituted before use. Store the vials at -80 ℃ after reconstitution.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Cerebral Cortex, Ambient temperature shipping, 10 ug/pk
Total RNA is isolated by modified guanidine thiocyanate techniques and stored in RNA storage buffer. The vials were then dried for room temperature shipping and storage. The total RNA need to be reconsitituted before use. Store the vials at -80 ℃ after reconstitution.
Norgen Biotek Corp RNase-Free DNase I Kit, 50 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.